The basis of the IAC measurement is the antigen-antibody interaction. All four main toxin antibodies are embedded throughout the column. After a sample is extracted and filtered, it is slowly passed through the IAC. The toxins bind to their corresponding antibody in the column. The IAC is then washed to remove unbound substances. The toxins are then eluted with the eluent, concentrated using nitrogen gas, then injected into an analytical instrument for detection. Affinity columns can be used in combination with HPLC and LC-MSMS to achieve rapid testing, and to increase signal-to-noise ratio and improve the accuracy of the detection method.

This kit is manufactured to the international quality standard ISO 9001.